Evaluation of Ceruloplasmin Oxidase Activity and C-Reactive Protein in the Sera of Patients With Diabetes Mellitus

N. Nasif, Zayzafoon

	Evaluation of Ceruloplasmin Oxidase Activity and C-Reactive Protein in the Sera of Patients With Diabetes Mellitus
Iraqi National Journal Of Chemistry
Article 1, Volume 0, Issue 37, 2014, Pages 175-185
Author
Zayzafoon N. Nasif
Abstract
Abstract The type two Diabetes Mellitus patients (independent DM2) was used to investigate the level of C-reactive protein CRP & ceruloplasmin oxidase activity CP. The study aimed to compare the effects of altering the glycemic index or the amount of carbohydrate (serum glucose) with C-reactive protein and CP activity in patients compare with control group. As a marker of systemic inflammation, raised CRP concentrations are still within the normal range have been associated with an increased inflammation of Diabetes Mellitus DM. The our experimental data reveal that ceruloplasmin oxidase enzymatic activity is a serum non-specific factor acting during the early and late stages of DM. Also this study aimed to compare the effect of raising blood sugar with levels of CRP & CP in DM2 patients. The Glucose kit method & CRP latex were used in present study, CRP kit which is based on immunological reaction between CRP antisera bound to biologically inert latex particles and CRP in the test specimens of patients with DM (13Female, 17Male median age years, range=30-65)yr & in control subjects (7F,14M ,age range=30-45) yr. The results are classified according to visible agglutination to: 1- A positive result / is indicated by the obvious agglutination pattern of the latex, in a clear solution. 2- A negative result: is indicated by no change in the latex suspension on the test slide. Then the results of the precipitin test correlated with the quantitative data on CRP. Ceruloplasmin oxidase activity measured by using the modified Rice method & phenylene diamine – 2HCl as a substrate . This study has found that CRP concentrations in patients with DM increased very clearly than normal subjects, and established that CRP conc. in male was more than in female for patients specimens. An increase in serum ceruloplasmin (CP) oxidase activity has also been indicated in both sex, higher activity noted in female than male. Abbreviations :CRP:C-reactive protein, mg/dl: milligram per diacelitter, BS:blood suger, CP: ceruloplasm in, F: Female, M: Male. DM2: Type Two Diabetes Mellitus

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