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TOXOPLASMA GONDII: EXPERIMENTAL INFECTION OF ISOLATED LOCAL STRAIN IN SULAIMANI PROVINCE | ||
| IRAQI JOURNAL OF MEDICAL SCIENCES | ||
| Article 1, Volume 6, Issue 3, 2008, Pages 60-71 | ||
| Authors | ||
| Shadan H. Abdulla; Lazem H. Al-Taie | ||
| Abstract | ||
| Background: Toxoplasma gondii infection in humans is widespread through out the world, approximately half a billion humans have antibody to T. gondii. Objective: The present study aimed to isolate and identified the local strain of Toxoplasma gondii from diaphragmatic muscles of naturally infected farm animals (sheep&goat) through studying the localization of the parasite in different organs of experimentally infected albino rats. Methods:The parasite was isolated from minced diaphragmatic muscles from naturally infected farm animal by digestion with acid–pepsin mixture .Fourteen albino rats were inoculated intraperitoneal with freshly prepared parasite, serodiagnosis was performed for all experimental rats after (6) weeks of inoculation using LAT. Post mortem examination and giemsa stained impression smears from internal organs were done at the end of the experiment. Results: Crescent or arc shaped with pointed one end and rounded other end, with typical gliding movements of tachyzoite was the important features of the parasite isolated from digested diaphragmatic muscles of farm animals .Impression smears from internal organs revealed presence of the parasite in,brain;lymph node; spleen; heart; liver; and kidney, and absent in,lung; peritoneum; uterus; and skeletal muscles. Conclusion: The parasite T. gondii was isolated successfully by acid -pepsine digestion procedure ;and experimentally infection.The most affected organs are, ,brain;lymph node;spleen; heart; liver; and kidney. | ||
| Keywords | ||
| Toxoplasma gondii; Isolation; Sulaimani; Acid; pepsine | ||
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