IN VITRO EFFECT OF PROTEINASE FROM C.ALBICANS ON L.DONOVANI

Th.Al-Marsome, Huda; A.F. Al-Attraqhchi, Azhar

	IN VITRO EFFECT OF PROTEINASE FROM C.ALBICANS ON L.DONOVANI
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 6, Issue 3, 2008, Pages 72-76
Authors
Huda Th.Al-Marsome; Azhar A.F. Al-Attraqhchi
Abstract
Background: Visceral leishmaniasis is a sever and often fatal disease caused by Leishmania donovani .Therapeutic failures have been reported either due to initial failure to respond to treatment or relapse. The extracellular proteolytic activity is one of the several hydrolytic activities described for Candida albicans. Acidic proteinase is an important virulent factor which improved it,s antibacterial activity. From this study we found that this enzyme has an anti leishmanial in vitro. Objective: To detect anti-leishmanial effect of proteinase enzyme produce by Candida albicans. Method: C.albicans standard strain (ATCC 10230) was cultured on wheat bran mediun PH 8.5 to produce proteinase enzyme in shaker incubater at 370C. The enzyme activity was determined by using 1% hemoglobin, pH 2.0, as a substrate16 .The enzyme was precipitated with 0.5% and 50-75% ammonium sulphate consecutively. Leishmania donovani (MHOM/IQ/82/BRC1) was used. Promastigotes were cultivated in RPMI medium, the number of parasites was adjusted to 1x106cell/ml. The crude enzyme (1ml) was added at different concentrations. Results: Intact proteinase and dilution of 1:2 were found to be effective on the growth of leishmania strain. Conclusion: Candidal proteinase enzyme have an anti leishmanial activity (promastigote stage) and this may be considered as a good clue for the probability of using this enzyme as treatment agent for this parasite after trying it,s activity on animal models.
Keywords
Candidal proteinase; donovani

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