VACCINE TRAIL AGAINST TRYPANOSOMA EVANSI

H. Al-Taie, Lazem

	VACCINE TRAIL AGAINST TRYPANOSOMA EVANSI
IRAQI JOURNAL OF MEDICAL SCIENCES
Article 1, Volume 6, Issue 3, 2008, Pages 77-84
Author
Lazem H. Al-Taie
Abstract
Back ground: Trypanosomiasis is one of the transmissible, zoonotic diseases that infect man & animals. Objectives: The present study was designed to investigate the immunization of adult Balb/mice with the immunomodulatoreLPG alone or mixed with NPGor BCG, by studying the hematological, immunopathological changes and immune response against Trypanosoma evansi infection. Methodes: 50 male Balb/c mice at (3-4) weeks of age divided in to 5 equal groups, animals of groups 1&2 were considered as control groups, animals of groups 3, 4&5 were injected 4 times i/p with equal dosage of different biological adjuvant as antigens: LPG, LPG+BCG, LPG+NPG; 20/0.08, 20/0.08+50/0.1, 20/0.08+50/0.25; μg/ml/mice, respectively. Immunized & +ve control animals were infected with trypanosome evanci by injection of parasite, experiment conducted for 12 days..Hematological parameters were determined using the MS9 .Immunopathological changes were refereed as liver and spleen weight /body weight Immune response detected by using IHA test. Results: Results revealed a significant variation in hematological, and liver &spleen weight between immunized infected, and non immunized infected animals. The biological adjuvant (LPG alone or mixed with BCG orNPG) had high immunogenic and less toxicity against the experimental infection of Trypanosoma evansi. Conclusion: The most successful immune responses (increase Ab) was in combination between LPG +BCG.While the more effective in decrease of severity of the disease(hematological & pathological changes)was in combination of LPG+NPG.
Keywords
Immunomodulaters; Trypanosoma evansi; Hematological and Pathological changes; IHA

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