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The Immunological effects of Entamoeba histolytica Antigens in White New Zealand Rabbits | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 2, Issue 2, 2009, Pages 67-81 PDF (233.38 K) | ||
| Authors | ||
| H. Sh. Al- Hayani; A. B. H. Al- Kubaisi; A. A. Shaker | ||
| Abstract | ||
| This study was conducted on 40 white New Zealand rabbits by injecting them with different Amoeba trophozoite antigens and the secretary and excretory products. The immunization with extract of amoebic trophozoite intraperotoneally against amoebic liver abscess gave a high protection (91.63)% as compared to intramuscular (88.43)% or subcutaneous routes (81.49)% respectively while immunization with the secretary and excretory products gave a protection of 54.13% against amoebic liver abscess, while the intramuscular and subcutaneous routes gave a protection of 48.77% and 38.14% respectively. There was a significant increase in the concentrations of antibodies type IgA , IgG (260 mg/ 100 ml, 1132 mg/ 100ml respectively) while there was no significant effect on the concentrations of antibodies type IgM (250 mg /100 ml). Results showed that immunization with trophozoite in muscle tissue gave a significant effect in increasing the concentrations of the complement C3 (171 mg/ 100 ml) and C4 (65 mg/ 100ml) Peritoneal immunizations had significant effects in reducing NBT stain. The percent of the cells forming formazan particles was (16.33) as compared to control group (6.67). Immunization with trophozoite, secretary excretory products, immunized serum and spleen cells showed a decrease in the liver and spleen weight. The highest decrease in the liver weight reached 2.92 when immunization had occurred with spleen cells intraperotoneally, while the highest reduction in the weight of spleen reached 0.04 when immunized with trophozoite extract and spleen cells through intramuscular and intraperotoneal routes. The Antigens of trophozoite stage gave a distinguished double–fold skin thickness (7.21, 6.13 mm) and erythema area (41.49, 46.41 mm2) after 24 and 48 hrs respectively. The same was true for secretary and excretory antigens that gave 5.36 , 5.12 mm double skin thickness and 31.34 , 34.63 mm2 for erythema area after 24 and 48 hrs. respectively , when hypersensitivity test was conducted. The viability percentages at the hour 204 In vitro were (9.33 , 22.33 and 25.67) % at the concentrations 0.5, 0.25 and 0.125 ml of immunized serum respectively. Immunized serum had a significant effect on killing E. histolytica trophozoite In vitro . It was concluded that immunization with amoebic antigens of E. histolytica reduces or limits the infection with amoebic liver abscess. | ||
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