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A Trying to produce an safety vaccine against Brucellosis | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 3, Issue 1, 2010, Pages 10-17 PDF (159.52 K) | ||
| Author | ||
| A. M. Hamzah | ||
| Abstract | ||
| In this study, a vaccine was prepared by production of several soluble antigens extracted from Rev1 strain of Brucella melitensis by sonicating the bacteria, and after cooling centrifuge the supernatant was discard while the extract passed through sephacryl G200 column to separate protein content according to its molecular weight. Four peaks were obtained which were used to induce cellular and humeral immunity by using five groups of mice (five mice/group) with control group. Each one peak injected subcutaneously to one group of mice at 100 µ/0.1 ml then giving one activated pooster dose after two weeks interval. A challenge was done by injecting intraperitonealy a virulent strain of Brucella melitensis of 1x610/0.1 bacteria/ ml after one month of vaccination. No bacteria was isolated from the vaccinated animal while isolate from control group. All four peaks uses as skin test in a group of mice after two weeks of vaccination with S19 vaccine and the best result obtained by the third peak. Cell mediate immunity was evaluated by using the skin test after two weeks from vaccination, where the third protein peak showed the best result in skin test and supported with histological examination results. Evaluate of humeral immunity by using of ELISA technique with Br.abortus Ag as coated sensitized antigen done on serum samples after two month of vaccination, also the third peak shown the best results. | ||
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