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Comparative Study to evaluate different programs of Newcastle disease vaccines in Broilers | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 3, Issue 1, 2010, Pages 72-79 PDF (131.31 K) | ||
| Authors | ||
| A. H. Shalash; Q. D. Lihaby | ||
| Abstract | ||
| Five programs were evaluated in this study comparison heamagglutination inhibition test (HI) with Antibodies which was evaluate by ELISA and protection rate after challenge. One hundred and twenty broiler chicks were divided to six groups and treated as following: Group1- vaccinated with live attenuated vaccine of Newcastle disease (LND vaccine) by spray method at (1,10,20) day of age. Group2- vaccinated with LND vaccine by spray method at (1) day of age and by drinking water method at (10, 20) day of age. Group3- vaccinated with LND vaccine by spray method and with oil adjuvant ND vaccine at (1) day of age, and with LND vaccine by spray at (10, 20) day of age. Group4- vaccinated with LND vaccine by spray method at (1,10, 20) day of age and with oil adjuvant ND vaccine at (7) day of age. Group5- vaccinated with bivalent vaccine of ND and infectious bronchitis (IB) at (1) day of age by spray method and LND vaccine at (10, 20) day of age by spray method . Group6- unvaccinated group as control . HI test carried out at (10, 20, 28) day of age while ELISA test at (20, 28) day of age, challenge test was carried out at (35) day of age to determinate the protection rate, the result was as following: there were significant differences in the titer of HI antibodies between the groups at (p≤0.01) that were G5 and G6 which gave the lower mean of antibodies while G4 gave higher mean of antibodies than G3, G1 and G2. There were significant differences of antibodies measured by ELISA at (p<0.01) G4 and G3 gave the highest titer than G1 and G2 compared with G5 that gave the lower titer like control group that gave negative test. In challenge test result G4 gave rate of protect (67%), G1and G3 (60 %) then G2 (50%) while in G5 and G6 the rate of mortality 100%. It was concluded that G4 which was vaccinated with LND vaccine by spray method at (1, 10, 20) day of age and oil adjuvant vaccine at (7) day of age gave the highest protective rate against challenge with virulent virus of ND. also it showed the negative effect of IB vaccine on ND antibody titer. | ||
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