Induction of True Estrous and Superovulation Using Licorice Extract (Glycyrrhiza glabra) in Local Iraqi Does

E. B. Redaa, F.; H. Al-Timimi, I.; M. Saleh, W.

	Induction of True Estrous and Superovulation Using Licorice Extract (Glycyrrhiza glabra) in Local Iraqi Does
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 3, Issue 2, 2010, Pages 76-88 PDF (0 K)
Authors
F. E. B. Redaa; I. H. Al-Timimi; W. M. Saleh
Abstract
This study was carried out in the animal farm of the college of veterinary medicine/Baghdad University. The effect of licorice extract, on reproductive performance of 40 mature local Iraqi does were studied, aged between 2.5-5 years, and three mature fertile bucks to observe does sexual behavior. The 1st experiment was conducted from 1-28 February 2008 on 25 does divided in to 4 groups which were treated weekly for 4 successive weeks with 300, 400 mg/Kg B.W. water solution of licorice extract weekly, 57 mg powder licorice extract with food/Kg B.W daily respectively with control group. Does were kept free for 4 hours daily with apronized bucks to observe their sexual behavior. Their weight and health were recorded. The aim of the 2nd experiments was to evaluate the effect of licorice extract in induction of estrous and superovulation in comparison with eCG in the period the sexual regression (from 1-27 march 2008) using vaginal sponges impregnated with 40mg MAP for 14 days in synchronization except the control group (Gc). The experiment was conducted on 28 does divided into 5 groups, (G1) and (G2) were treated with 300mg and 400mg/Kg B.W. of licorice extract respectively, (G3) injected with 500 IU of eCG IM 48 hr before removal of the sponges, (G4) treated with normal slain 48 hr before removal of the sponges. Does were left with apronized bucks to observe their sexual behavior for 7 days then, Exploratory laparotomies were done for does of each group to record the gross changes on ovaries and genitalia. The 3nd experiment was conducted to evaluate the effect of licorice extract (in 2 different times of administration) in induction of estrous and superovulation at the sexual regression period (7 April – 9 May 2008). In this experiment 30 does divided into 5 groups, estrous cycle of all groups had been synchronized with vaginal sponges except in (Gc). G1 was treated with 300mg licorice extract/ Kg BW. 28 hr before removal of the sponges, G2 was treated with 300mg licorice extract/ Kg BW, G3 treated with 500 IU eCG at the day of removal of the sponges and G4 was treated with 300mg licorice extract/Kg BW + 500 IU eCG at the day of removal of the sponges. Does were left with apronized bucks to observe their sexual behavior for 7 days then, Exploratory laparotomies were done for two does showed of signs estrous signs from each group. In the 1st experiment, results showed the effect of licorice extract on does reproductive performance, include the results were recorded 83.3%, 50%, 33.3% estrus percentage for does treated with 400 mg, 300 mg water solution of licorice/ kg B.W. weekly and 57mg powder of licorice/ kg B.W daily respectively, as compared to 28.5% for controls at the end of breeding season. The results of 2nd experiment were recorded on estrus induction, 50% for groups which had treated with 300 and 400mg licorice/kg B.W and 60% for 500 IU eCG, before 48 hrs of sponges removal, as compared to 0% control, with 4,1,1 prolificacy of 1st three groups outside of breeding season. In the 3rd experiment, groups treated with 300 mg/Kg licorice solution 48 hrs of sponge’s removal, 500 IU eCG and 300 mg licorice/kg + 500 IU eCG at the time of removal of sponges, showed that induction of estrous and superovulation were higher than those treated with 300 mg water solution of licorice at the time of removal of sponges and the control group. Group treated with 300 mg licorice/kg + 500 IU eCG showed high ovarian activity and ovulation rate, ovarian size was 35x30mm, these ovaries known as (Herculean ovary), and the number of ovum was ranged from 3-13 ovum/doe as recorded by laboratory. It was concluded that licorice can be used for induction of estrus and superovulation in does.

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