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Co-cultivation of cell suspension derived from leaf callus of Dianthus caryophyllus with Ti plasmids | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 15, Issue 1, 2010, Pages 68-73 | ||
| Author | ||
| Q. Sh. Al-Ne | ||
| Abstract | ||
| Ti-plasmid (Tumor inducing plasmid) was isolated from Agrobacterium tumefaciens Binn A6. Cell suspension was established from leaves callus of Dianthus caryophyllus in liquid Murashige and Skoog (MS) medium supplemented with 0.5 mg / L 2,4-D and 0.1 mg / L BA. The favorite density were ( 383 × 103 and 420 × 103 cell / ml). They mixed with certain volum of Ti-plasmid (20, 50 and 70 µL), the mixture cultured by multiple drops of agar. The results indicate that mixing Ti-plasmid with cell suspension enhanced the formation of cellular colonies, and were found to increase the number of callus primordia formed. | ||
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