Isolation and Identification of Fungi from Peas seeds and its Biochemical Resistance

S. Alinizy, Ghaydaa

	Isolation and Identification of Fungi from Peas seeds and its Biochemical Resistance
Tikrit Journal of Pure Science
Article 1, Volume 15, Issue 1, 2010, Pages 74-80
Author
Ghaydaa S. Alinizy
Abstract
The Result of this experiment show that Peas’ seed safety tests revealed six fungi species. Rhizopus species was the most frequent isolated species with frequency rate of 52, followed by Penicillium fungus 36, Aspergillus niger 19, while the frequency rate of both Aspergillus flavus and Rhizoctonia solani was 8 isolations for the four varieties. The least frequent fungus species was Stemphylium spp. This fungus was isolated from Lincoln variety only, and didn’t appear in any of the four varieties which tested in this study. Rhizoctonia solani fungus inhibited seed growth rate by various percentages; highest inhibitory percentage reached to 28% for Lincoln and Alaska varieties, meanwhile the lowest inhibitory percentage was 18% for the local varieties. Dianthus oil had the inhibitoriest effect on fungus growth, with inhibitory rate reaching 100% for the four Dianthus oil concentrations used, followed by Cinnamon oil with fungus growth inhibition range of 100% at 100:1 concentration to 100% at 50:1 and 25:1 concentrations. Myrtle oil was the least effective in growth inhibition, with inhibition rate of 68.7% at 100.1 concentration, and 87%.5 at 25:1 concentration, the inhibition rate increased with the increase of oil concentration. The Herbicide used was able to inhibit the growth of Rhizoctonia solani fungus by 100%. Fungi bio-resistance tests using Bacillus subtilis bacteria showed the inhibition of R.solani Fungus by 100%.

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