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Inhibition the Growth of Some Malignant Cells Lines by Alfalfa Aqueous Extract | ||
| Iraqi Journal of Cancer and Medical Genetics | ||
| Article 1, Volume 3, Issue 2, 2010, Pages 46-50 | ||
| Author | ||
| Rafid abdul.wahid.A | ||
| Abstract | ||
| The aim of the present study was to assess the cytotoxic activity of aqueous extracts of Alfalfa (Medicago sativa ) by using (MTT) (colorimetric assay) to measure the cytotoxic activity on two malignant tumor cell lines (AMN3 ) Ahmed Mohamed Nahi 2003 and (GB) Glioblastoma cell line ,and one type of normal cell line (MEF) Mouse Embryo Fibroblast cell line, through different exposure time (24, 48 ,and 72hrs ) and different concentration of aqueous extract ; (1000,500,250,125 ,and 62.5, mg/ml), the study was carried out in the Iraqi Center for Cancer and Medical Genetics Research ,The result showed that the aqueous extracts from alfalfa have significant effects P value ≤ 0.05 on the growth of AMN3 cell lines and GB malignant cell line in culture in dose and time- dependant manner ,Viability of cell lines decreased with time and concentration reaching its lowest value after 72 hrs of treatment with the highest concentration used (1000 mg/ml ). The results also indicated that GB cell lines were more sensitive to the Alfalfa aqueous extracts as compared with the growth of AMN3 cell lines . From the other side the result showed that there is no significant effect P value ≥ 0.05 of these aqueous extracts on the viability of normal cell line (MEF3 cell line). Only the treatment with higher concentration (1000μg/ml) after 72 hrs caused significant reduction P value ≤ 0.05 in the (MEF) cell line growth. | ||
| Keywords | ||
| Alfalfa; aqueous extracts; anticytotoxic activity; Mtt assay | ||
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