NEW STAINING SOLUTION FOR  IDENTIFICATION OF HELICOBACTER PYLORI IN THE GASTRIC TISSUE

Kassim Hussaen, Yahya; Ali Muhammaed, Najah; Abid Al-satar, Shahrazad

	NEW STAINING SOLUTION FOR IDENTIFICATION OF HELICOBACTER PYLORI IN THE GASTRIC TISSUE
AL-TAQANI
Article 1, Volume 22, Issue 2, 2009, Pages 136-142
Authors
Yahya Kassim Hussaen; Najah Ali Muhammaed; Shahrazad Abid Al-satar
Abstract
In this study an attempt was made to study the efficiency of the synthetic Brielliant blue food color powder color index (C-1. No 42090) for identification of Helicobacter pylori (H.pylori) in gastric tissue. In this study 3 types of staining solutions were prepared; each type includes 3 series of concentrations 2.5%, 5% and 10%. 1st type of staining solution consist of Brielliant blue food color powder dissolved in distilled water the 2nd type consist of blue color powder dissolved in 0.5% of aqueous solution of borax, and the 3rd type was consist of blue color powder dissolved in 0.5% of aqueous solution of boric acid. The gastric tissue sections were stained with 3 concentrations of each type of the staining solutions and with Hematoxylin and Eosin stain as a control stain. Best result for staining of H.pylori, was obtained by using the third type of staining solution with 10% concentration, these organisms were showed with dark blue color, this made their identification very easy in the gastric tissue, whereas this solution wasn't efficient for histological examination. After following up, the stained bacterial cells, it was observed to be stable in color for 18 months. According to these results, 10% concentration of the third type staining solution may be considered as an efficient staining solution for identification of H.pylori in the gastric tissue.
Keywords
Synthetic Brilliant blue food color powder; Helicobacter Pylori

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