Hemagglutination properties of some intestinal bacterial pathogens isolated from clinical samples

Ali Shareef, Hager; Tajer Abdulla, Eman; Najat Mostafa, Zubaidah

	Hemagglutination properties of some intestinal bacterial pathogens isolated from clinical samples
Tikrit Journal of Pure Science
Article 1, Volume 15, Issue 3, 2010, Pages 5-10
Authors
Hager Ali Shareef; Eman Tajer Abdulla; Zubaidah Najat mostafa
Abstract
Hemagglutinating properties of four intestinal bacterial pathogens(Escherichia coli , Salmonella sp. , Klebsiella sp. , and Proteus sp. )isolated from human clinical samples in Pediateric hospital at Kirkuk city were determined. Red blood cells of different origins as human blood group A,B,AB and O,Sheep, and Chicken were used in this study. Bacterial genera cultured in different solid media at 37c like Tryptose soy agar (TSA) and broth ,Brian heart infusion agar (BHI) and broth , and Luria Bertani agar (LBA) broth. The results showed that all bacterial isolates were able to express hemagglutinating activitywith human blood group A,B,and O and Sheep and Chickenerythrocyte, and this expression was better in isolates cultured on TSA agar than other exmined media. For the detection of Mannose – sensitive and mannose –resistant hemagglutination (HA), reactions were run with and without 1% (w/v) D-mannose, all bacterial genera grown on TSA produced two main kinds of hemagglutinins ; (a) mannose resistance hemagglutination (MRHA) of erythrocyte from human bloods group B and O,Sheep and Chicken ; (b) mannose sensitive hemagglutination(MSHA) of erythrocyte from human blood group A . Other sugars like D-mannitol, D- glucose ,D- maltose, D-galactose, D-fructose, D- xylose, and Dulcitol ,was tested in this study and we observed that all these sugars caused inhibition of hemagglutination of human blood group B erythrocyte by those organisms whereas they were not inhibitory to human group AandO, Sheep and Chicken erythrocytes.

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