The Study of the Effect of Acidophilic Milk on Enterobacteriaceae in Normal Flora of White Mice

	The Study of the Effect of Acidophilic Milk on Enterobacteriaceae in Normal Flora of White Mice
Tikrit Journal of Pure Science
Article 1, Volume 15, Issue 3, 2010, Pages 146-153
Abstract
Twenty five isolates of Lactobacillus acidophilus (LA) were obtained from pickled vegetables (16) , dairy (2) and from mice gastrointestinal (7), which were by using morphological, cultural and biochemical tests. All the Strians of isolates had been tested for bacteriocin production. three of them were positive for this test done by well diffusion methods. The best activity of bacteriocin was at pH (5 -6) and the highest stability was at the 5 pH. The inhibitory activity of bacteriocin produced from Lactobacillus acidophilus was greater than Staphylococcus aureus and Lactobacillus plantarum, as compared to the effect on Escherichia coli. This study proves that acidophilic milk has a clear effected on decreasing the number of Enterobacteriaceae in mice gastrointestinal from (4.9 cfu/ml) to (3 cfu/ml). The Lactobacillus acidophilus which isolated from mice gastrointestinal is beter than those isolated from pickeld vegetables to decrease the number of Enterobacteriaceae. There was a positive effect for acidophilic milk on increasing the number of Lactobacillus acidophilus in mice gastrointestinal from (3 cfu/ml) to (4.6 cfu/ml). There was no significant effect for sterilized acidophilic milk (no bacterial growth) on Lactobacillus acidophilus number.

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