Assessment of genomic instability in blood and tissues from prostate cancer patients by random amplified  polymorphic DNA analysis

H. Ghali, Zafir; H. Ahmed, Intisar; K. Shubber, Esmail; A. Al-Ani, Badry; K. Sulaiman, Bilal; A, A; Shaima,

	Assessment of genomic instability in blood and tissues from prostate cancer patients by random amplified polymorphic DNA analysis
Jornal of Biotechnology Research Center
Article 1, Volume 3, Issue 2, 2009, Pages 30-39
Authors
Zafir H. Ghali; Intisar H. Ahmed; Esmail K. Shubber; Badry A. Al-Ani; Bilal K. Sulaiman; a A;
Abstract
All genetic alterations in the genome that predispose to the development of cancer are utmost importance in gaining a complete understanding of the exact molecular events involved in the development of tumorigenesis. In this study, the random amplified polymorphic DNA (RAPD) technique was used for assessing genomic instability in prostate cancer patients. DNAs were obtained from blood and tumor tissues of five prostate cancer patients, then amplified individually by RAPD with five different 10-mer arbitrary primers. The ability to detect genomic instability in five cancer tissues by each primer ranged from 20 to 100 percent. Change in the genome that revealed by RAPD technique included deletion or insertion and allelic losses or gains. The most important finding is that deletions were also observed in blood DNAs, while the corresponding fragments were present in the tumor DNA. Our results display an insertion of a 1093 bp fragment in 2 of 5 tumor samples using primer OPA-03. A deletion of the same 1093 bp fragment was observed in a blood sample using the primer OPA-03. These results confirm other reports that RAPD technique is useful for assessing genomic damage in cancer

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