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Effect of Extract Callus Plant Rue on the Growth of Bacteria Sinorhizobium meliloti and its Relationship with Plant Alfalfa Medicago sativa | ||
| Rafidain Journal of Science | ||
| Article 6, Volume 21, Issue 7, 2010, Pages 78-86 PDF (0 K) | ||
| DOI: 10.33899/rjs.2010.35129 | ||
| Authors | ||
| Fawz A. Al-Safar; Mira A. Al-Katib; Najwa I. Al- Barhawi | ||
| Abstract | ||
| The disparate impact of extract callus plant Ruta graveolens L.( rue) on the growth of bacteria Sinorhizobium meliloti in the nutrient medium, as noted its effect inhibitory on the growth of bacteria in the medium of Manitol Salt Yeast Extract (MSY) liquid in terms of numbers tend to decrease by 47.5 and 38.6%, as shown by its effects as an energy source when you add it to the medium of nutritional deficiencies Rhizobium Minimal Mediam (RMM) instead of sugar Mannitol and increasing the bacteria by 27.7 and 12.3%, after 24 and 48 hours of incubation compared to a sample comparison (bacteria developing in the nutrient medium without the addition of extract), in terms of measuring the turbidity at the spectral wavelength 595 nm,in the other hand, this extract inhibited the formation of root nodules on alfalfa seedlings inoculated with the bacterium S. meliloti developing in the middle (NF) Nitrogen Free solid, also reduced the number of vegetative branches , and leaves, with the death of some of seedlings and low protein content, but at the same time, led to a higher level in the inoculated seedlings compared to sample comparison | ||
| Keywords | ||
| meliloti Sinorhizobium | ||
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