The Effect of Sperm Preparation Medium on Malondialdehyde Concentration in the Seminal Plasma of Normozoospermic and Oligoastheno- teratozoospermic patient

Khalid Abd-Al Kareem, Maha

	The Effect of Sperm Preparation Medium on Malondialdehyde Concentration in the Seminal Plasma of Normozoospermic and Oligoastheno- teratozoospermic patient
Jornal of Biotechnology Research Center
Article 1, Volume 3, Issue 2, 2009, Pages 74-79
Author
Maha Khalid Abd-Al Kareem
Abstract
Intra uterine insemination (IUI) has been widely used to treat infertility. Infertility may be due to increased levels of reactive oxygen species (ROS) in the seminal plasma. The goal of our study was to confirm that using sperm preparation medium to activate the spermatozoa in IUI might be helpful to decrease the levels of ROS in the seminal plasma, lowering the oxidative stress and the damage effects of the sperm and enhance a good quality sperm capable of capacitation, acrosomal reaction and fertilization. The levels of malondialdehyde (MDA) were measured in the seminal plasma in 40 infertile men of normozoospermia and oligoastheno-teratozoospermia before and after adding medium to the samples. Results showed that there was a significant (P<0.05) decrease in all parameters of semen samples in oligoastheno-teratozoosperia as compared with the other group, there was no significant increase in the level of MDA before adding the medium to the samples and a significant (P<0.05) increase after adding the medium to the same samples in the second group as compared with normozoospermic patients. Results reported in this study, clearly indicated that adding sperm preparation medium to the semen samples would improve the fertilization rates by decreasing the oxidative damages to the spermatozoa. Our results might be need a further studies to find a clue for the treatment of infertility in man.

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