EXTRACTION AND PURIFICATION OF COLICIN PRODUCED BYESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION

Falih Hassen AL- Dhumaina, Talib

	EXTRACTION AND PURIFICATION OF COLICIN PRODUCED BYESCHERICHIA COLI ISOLATED FROM URINARY TRACT INFECTION
AL-TAQANI
Article 1, Volume 22, Issue 4, 2009, Pages 73-81
Author
Talib Falih Hassen AL- Dhumaina
Abstract
1017 urine samples were cultured from patients suffering from urinary tract infection. Out of these 648 isolates were obtained and 369 gave no bacterial growth . E. coli showed the high percentage 42.1% followed by Proteus mirabilis 12% ,Klebsiella pnuemoniae 8.7%, Psuedomonas aeruginosa 7%, and Enterobacter spp 6.3% . While the percentage of Gram positive bacteria (Staphylococcus aureus, Streptococcus pnuemoniae and Staphylococcus saprophyticus were 10,1%,8.3%and 5% respectively. The local E. coli isolates from UTI were examined for colicin production by exposing each isolate to the rest of all others and looking for the inhibition of the growth, three methods for this test were employed , cup assay , wells assay and cross streaking , Cup assay was found to be the most efficient , thus was used throughout the study. Six types of modified media were used to choose the best one ( give highest production of colicin , brain heart with 5% glycerol was the most efficient medium for colicin production . colicin was extraction by homogenization with NaCl which gave an activity 640 U ml. Purification of crude colicin had been carried out by using ammonium sulphate result indicated that (40-60) was regarded as the best saturation ratio for colicin precipitation , the specific activity of colicin after the dialysis was 1066.6. The activity spectrum of purified colicin was studied and found to affect the isolates which caused UTI.

Statistics Article View: 111 PDF Download: 38