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Purification of algnic acid by Local isolate of Azotobacter Vinelandii | ||
| Ibn Al-Haitham Journal For Pure And Applied Science | ||
| Article 1, Volume 22, Issue 2, 2009, Pages 39-46 | ||
| Abstract | ||
| The bacteria Azotobacter Vinelandii was taken from a central research in Baghdad, The purification of alginic acid which produced from the bacteria by several steps starting with precipitation with isopropanol (3:1) v/v , Washing by ppt with 100ml of isopropanol : distilled water (3:1) v/v , then the ppt was dissolved in warm distilled water and dialysis against distilled water from 24 h/s . To Complete the purification , gel filtration chromatography was conducted on sephacryl s-100 column followed by ion – exchange chromatography . Using DEAE cellulose column . The molecular Weight of purified al ginic acid was higher than that of blue dextran 2000,It was more than (2) millions Dalton . | ||
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