Total bacterial count , total acidity and pH were determined in four baby milk formlae Dielac 2(D 2 ) , Dielac 1( D 1 ) , Babylack 2( B2 ), Babylack 1( B1 ) after they were in inoculated with two local isolates ( 3 , 4 ) of Bifidobacterium ssp. In concentration 2% .It was noted that the number of these two isolates increased in first baby ages formlae that those of the second ages formlae from ( 2.0 × 1012 -3.2 × 1014)
( 5.6 × 1013 – 2.5 × 1016) , ( 2.4 ×1010 – 1.2 × 1014) , ( 2.5 ×1012 – 1.4 × 1013) , ( 1.4 × 1013 – 2.4 × 1015) , ( 1.9 ×1011 – 9.8 × 1013) , ( 5.1 × 1012 – 9.8 × 1014) and ( 5.0 × 1012 – 2.7 × 1014) cfu/ g respectively and the total acidity isolate (3) ranged between (0.08 -0.35) , (0.09 – 0.30 ) , (0.10 – 0.40 ) , (0.08 – 0.38 ) , respectively. Whereas pH was dropped down from 6.60 , 9.50 ,6.40 and 6.60 to 4.50 ,4.60 ,4.70 and 4.70 in formulae D1 , B1 , D2 and B2 respectively. Results of isolate ( 4 ) in four formulaes, were (6.60 - 4.00 ) , (6.95 -4.50 ) , ( 6.40 – 4.20 ) , and (6.60 – 4.70 ) for pH , while the total acidity were ( 0.08 – 0.45 ) , ( 0.03 – 0.33 ) , ( 0.10 – 0.55 ) and ( 0.08 – 0.47 ) respectively . it was found that both of two isolates under study have good inhibition activity against E.coli and S.aureus as their counts were decreased from 106 c.f.u / g to 2 and 5 c.f.u / g in D1 , B1 , D2 and B2 respectively , also S.aureus number were decreased to 0 , 0 , 0 and 3 c.f.u / g D1 , B1 , D2 and B2 respectively . |