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DETECTION OF POLYMORPHISM OF HYDATID CYSTS PARASITE ISOLATED FOR DIFFERENT REGIONS OF IRAQ USING PCR TECHNIQUES | ||
| AL-TAQANI | ||
| Article 1, Volume 22, Issue 1, 2009, Pages 0-0 | ||
| Abstract | ||
| This research includes a study of hydatid cysts parasite on the molecular level where 20 samples of hydatid cyst were collected from intermediates host (sheep) for different geographical regions of Iraq. Genetic analysis of isolated DNA from hydatid cyst collected from sheep was done by polymerase chain reaction (PCR)to determine genetic variation depending on random amplified polymorphic DNA "RAPD" . The current results of this study have shown the following: 1. It was found the primer OPF-19 was able to diagnose samples the represent the isolated DNA of hydatid cysts parasite which was collected from sheep in intermediates regions of Iraq by genetic marker OPF-19 1050bp . 2. Ability of RAPD markers to determine markers of OPF-16 65obp specified to samples of hydatid cysts DNA which was collected from sheep in northern regions of Iraq , and another marker which was OPF-16 500bp specific for samples collected from sheep in northern regions of Iraq . 3. Ability of RAPD markers to determine two markers OPF – 6 770 bp , 460pb for samples which was collected from hydatid cysts of sheep in Intermediat regions of Iraq,and other marker which was OPF -6 650bp specific for samples collected form hydatid cysts of sheep in Southern regions of Iraq . | ||
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