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Spectrophotometric Determination of Nitrazepam by Coupling of it's Diazotized Reduced form with m- Aminophenol as Coupling Reagent | ||
| Rafidain Journal of Science | ||
| Article 9, Volume 21, Issue 7, 2010, Pages 123-140 PDF (0 K) | ||
| DOI: 10.33899/rjs.2010.36059 | ||
| Author | ||
| Maha T. Hussin | ||
| Abstract | ||
| This paper describes the development of a spectrophotometric method for the determination of microgram amounts of nitrazepam after diazotization of it’s reduced product and coupling with m-aminophenol in basic medium, to form an intense orange – coloured, water – soluble and stable azo-dye which shows a maximum absorption at 478 nm. Beer’s law is obeyed over the concentration range of 5 to 200µg/25ml with a molar absorptivity 17581.25 l. mol-1.cm-1 and Sandell’s sensitivity of 0.016 µg.cm-2. The relative error is ranged from -2.11 to +1. 4% and relative standard deviation from ±0.55 to ±2.10 %, depending on the concentration level. The method has been applied successfully to the determination of nitrazepam in pharmaceutical preparation (tablet). | ||
| Keywords | ||
| nitrazepam; reduction; diazotisation; aminophenol; spectrophotometry | ||
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