A Study of Toxic Effects of Aspergillus flavus on Total Leucocytes Count             Measurement (W.B.Cs) and Histological Parameters of Female Albino Rates and Capacity of Biological Control on this Effect .

	A Study of Toxic Effects of Aspergillus flavus on Total Leucocytes Count Measurement (W.B.Cs) and Histological Parameters of Female Albino Rates and Capacity of Biological Control on this Effect .
Magazine of Al-Kufa University for Biology
Article 1, Volume 2, Issue 1, 2010, Pages 84-96
Abstract
Aims Of study : This study was executed in laboratories of biology department in the college of science it clarifies the toxic effects of the two fungi isolated from the Aspergillus flavus it one ability to produce the aflatoxin but others do not produced it, on some physiological, and histological parameters of white rat female. In addition to evaluation of the efficiency the bioformulation from the Bacillus subtilis bacteria in protecting the animals food from the infection by the fungi A. flavus. The laboratory experiments proved that the The laboratory experiments proved the bioformulation from the B. subtilis had a high effect in inhibition the radial growth in concentration 1 g / L where the inhibition rate attained 100 % to the both fungi isoluted from the A. flavus . The result of this study demonstrated the presence of different toxic effect from the both fungi isolated from the A. flavus by increasing the level of white blood cells to about 10400 cell / µm3 in rats that injected with filtration of the isolated which produced of the aflatoxin , and 6300 cell / µm3 in rats that injected with filtration from other isolated of the A. flavus compared with of control 3400 cell / µm3 . On the other hand the histological study showed that fungi isolated which produced to the aflatoxin but not treated with bioformulation of the B. subtilis it caused pathological effects on some tissue of the laboratory rats these effects included vascular conducation in the liver tissue , and clear necrosis in some of the liver cells also it causes villous atrophy and characterized by the presence of inflammatory cells and destruction of the mucosa in the small intestine tissue .But the other isolated of the fungi A. flavus dose not caused any pathological effect . also the bioformulation of the B. subtilis it very efficient in the protection the liver and small intestine tissue from the effects of the aflatoxin .This results are considered the primary in Iraq according to the presence scientific references .

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