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New Analytical Method for Determination Chondroitin Sulfate by Ion Exchange | ||
| Journal of College of Education | ||
| Article 1, Volume 0, Issue 1, 2017, Pages 331-343 | ||
| Authors | ||
| Lamia Lafta Gashim; Arwa Mahmood Hussen | ||
| Abstract | ||
| We performed the quantitative analysis of chondroitin sulfate (CS) obtained from tablets.To quantify CS content in tablets ,each test sample was first extracted with hexane followed by phenol-chloroform to remove oil and various ingredients . The extracted CS samples were depolymerized by acid .The CS disaccharides produced were analyzed by strong anion-exchanger and CS content was quantified by calculating the amount of iodate (IO-3) found in amixture after adisaccharide compound has been oxidized with periodate (IO-4) . Quantitative analysis of the disaccharides drived from tablets showed the CS contents were 96 %. In conclusion , the quantitative analysis of the disaccharides produced by acid digestion can be used in the direct quantitation of CS in pharmaceutical preparations . | ||
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