IN VITRO MICROPROPACATION OF Lilium longiflorum

I. Mohammed, Haider; H. Taha, Fadia; K.M. Ameen, Sami

	IN VITRO MICROPROPACATION OF Lilium longiflorum
Diyala Agricultural Sciences Journal
Article 1, Volume 3, Issue 1, 2011, Pages 252-268
Authors
Haider I. Mohammed; Fadia H. Taha; Sami K.M. Ameen
Abstract
A study on effect of several factors on in vitro micropropagation of Lilium longiflorum was conducted in the plant tissue culture Lab./Hort. Dept./ College of Agric./ Uni. Of Baghdad from Oct.2009 to June 2010. The experiments were carried out to improve shoot multiplication and rooting . Seculent leaves were separated from the lilium bulbs , they were surface sterilized and cultured on MS medium . Multiplication experiments included ;using modified MS with 0 , 1.0, 2.0, 4.0 or 8.0 mg/l of BA or and 0,0.2, 0.4or 0.8 mg/l of CPPU .The second experiment was modifying MS medium with 0,0.2, 0.4 or 0.8 mg/l of NAA to the medium which contained the concentrations of BA or CPPU mentioned above . Rooting experiments were ; shoots cultured on MS medium supplement with 0,0.2, 0.4 or 0.8 mg/l of NAA ; 1/2 MS salt strength modified with 20 , 30 or 40 g/l of sucrose ; the last rooting experiment was supplementing 1/2 MS salt strength with 0,0.2, 0.4 or 0.8 mg/l of NAA . The results can be summarized as follows : BA levels reduced No. of shoot/ explants and length of shoots , while CPPU at 0.4 mg/l enhanced shoot multiplication and No. of leaves/ shoot . High concentration of sucrose increased rooting % and no. and length of roots/shoot . Reducing MS salt strength to a half was not effective on rooting .
Keywords
Lilium longiflorum; VITRO MICROPROPACATION

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