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Study of optimum conditions for uricase enzyme production by Pseudomonas aeruginosa | ||
| karbala journal of pharmaceutical sciences | ||
| Article 23, Volume 2, Issue 2, 2011, Pages 264-278 PDF (0 K) | ||
| Authors | ||
| Etab Abdul Ameer Al-Mosawi; Sahib Ali Mahdi Al-Atraqchi | ||
| Abstract | ||
| The ability of 15 bacterial isolates were tested to production uricase enzyme , by using the solid and submerged culture in the primary and secondary screening . The isolate P.aeruginosa 7 was selected for it's best in production of enzyme . the optimum fermentation condition for uricase enzyme production by P. aeruginosa 7 were examined . Results showed that used 0.05% syrup dates as carbon source, and used o.3% from uric as inducer , 0.75% from KH2PO4 , 0.1% from Mgcl2 . The other optimum condition for production of uricase enzyme were selected , the optimum pH was 6.5 and incubation period was after 24 hrs. , and the optimum temperature 37 CO . However the shaking was at 150 rpm , and inoculum volume was 1 from culture volume . | ||
| Keywords | ||
| studa; uricase enzy | ||
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