Ahmed Najy, N., Ismaeel Yaseen, A., Asra, , Ghali Al-Salihi, F. (2011). Estimation of alkaline phosphatase from salivaof diabetic patients and partial purification of it's isoenzymes. , 2(2), 279-289.
Nazar Ahmed Najy; a Ismaeel Yaseen; Asra; Ferah Ghali Al-Salihi. "Estimation of alkaline phosphatase from salivaof diabetic patients and partial purification of it's isoenzymes". , 2, 2, 2011, 279-289.
Ahmed Najy, N., Ismaeel Yaseen, A., Asra, , Ghali Al-Salihi, F. (2011). 'Estimation of alkaline phosphatase from salivaof diabetic patients and partial purification of it's isoenzymes', , 2(2), pp. 279-289.
Ahmed Najy, N., Ismaeel Yaseen, A., Asra, , Ghali Al-Salihi, F. Estimation of alkaline phosphatase from salivaof diabetic patients and partial purification of it's isoenzymes. , 2011; 2(2): 279-289.

Estimation of alkaline phosphatase from salivaof diabetic patients and partial purification of it's isoenzymes

karbala journal of pharmaceutical sciences
Article 24, Volume 2, Issue 2, 2011, Pages 279-289    PDF (0 K)
Authors
Nazar Ahmed Najy; a Ismaeel Yaseen; ; Ferah Ghali Al-Salihi
Abstract
This study was performed on 181 saliva specimens of diabetic patients ( 59 of type 1 & 122 of type 2 ), in addition 149 healthy saliva specimens were investigated as control group. The result of this study showed a significant increase (p=0.000) in activity of salivary alkaline phosphatase from diabetic patients (both types ) compared to healthy subjects.
Three isoenzymes were isolated from saiva of diabetic patients by addition of ammonium sulphate ,dialysis and ion-exchange (DEAE-Cellulose A-50) ,Isoenzyme I was partially purified by gel –filteration using Sephadex G-100 and showed a single band through electrophoresis by using SDS-PAGE and Coomassie blue R250 dye.
Keywords
alkaline phosphatase; diabetic patients
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