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Spectrophotometric Determination of Vitamin B6 by Coupling with Diazotized p-Nitroaniline | ||
| Rafidain Journal of Science | ||
| Article 6, Volume 21, Issue 8, 2010, Pages 49-59 PDF (0 K) | ||
| DOI: 10.33899/rjs.2010.36845 | ||
| Author | ||
| Asma N. Abdul Kadir | ||
| Abstract | ||
| A spectrophotometric method has been described for the determination of pyridoxine hydrochloride (vitamin B6) in pure and pharmaceutical preparations. The method is based on coupling reaction of vitamin B6 with diazotized p-nitroaniline in alkaline solution in the presence of CTAB to form an azo dye which has maximum absorption at 480 nm. Beer's law is obeyed over a range of 5-500g/25ml with a molar absorpitivity of 2.7 × 104 l. mol-1.cm-1 and Sandell sensitivity index of 0.0076 g.cm-2, a relative error +0.22 to + 0.47% and a relative standard deviation of ± 0.11 to ± 0.98%, depending on the concentration level. The proposed method has been applied successfully to the determination of vitamin B6 in pharmactical preparations. | ||
| Keywords | ||
| spectrophotometry; diazo; nitroaniline; vitamin B | ||
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