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Determination of Catechol Using Flow Injection -Electrochemical Detection with Banana Paste Electrode: Application to Adrenaline Determination | ||
| Rafidain Journal of Science | ||
| Article 7, Volume 21, Issue 8, 2010, Pages 60-70 PDF (0 K) | ||
| DOI: 10.33899/rjs.2010.36862 | ||
| Authors | ||
| Yousra M. Al. Shaker; Amer T. Al-Taee; Wadala A. Bashir | ||
| Abstract | ||
| This work involves indirect determination of catechol compounds using flow-injection electrochemical detector. The detector based on the three-electrodes system, banana paste [which contained the polyphenyl oxidase (PPO) enzyme] as working electrode, auxiliary electrode Pt wire and silver/silver chloride, saturated potassium chloride Ag/AgCl .sat.KCl as reference electrode. The PPO enzyme in banana paste motivates the oxidation of the two hydroxyl groups in catechol to the o-quinone which is then reduced at(-0.65)V back to catechol. The reduction current was recorded and it is proportional to concentration of catechol. The technique has been applied to adrenaline hormone | ||
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