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Isolation of Bacillus spp. from some sources and study of its proteolytic activity | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 16, Issue 4, 2011, Pages 59-63 | ||
| Author | ||
| May A. A. Al-Allaf | ||
| Abstract | ||
| In view of the importance sources that employ in our study that deals with human poisoning , this study was aimed to isolate Bacillus spp. from some sources that deals with human food poisoning and to identify this microorganisms morphologically and biochemically . Twelve samples including ( air , water , egg , milk , cheese and meat ) in duplicate were taken in this study , after isolation of the Bacillus spp. from these samples an investigation was taken up to find out the activity of proteolytic enzymes by this bacteria under controlled condition as one of its important virulence factors . The results showed that all the sources were contaminated with Bacillus spp. And the average numbers of colonies were found to be more in air samples followed by meat , milk , egg , water and the lowest in cheese samples. Also all the colonies of Bacillus which isolated were positive to proteolysis activity by forming clearing zones around the growth of the bacteria , because of its ability to degradation proteins to amino acids | ||
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