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Limited Normal Range Of Rhodanese Enzyme by Spectrophotometric method | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 16, Issue 3, 2011, Pages 141-145 | ||
| Authors | ||
| Israa A.Aljarah; Thikra A.Allwsh | ||
| Abstract | ||
| Rhodanese enzyme activity in (whole blood, plasma, serum, hemolysis red blood cell) was studied and shown higher enzyme activity in hemolysis Red Blood Cell and found no significant difference in rhodanese activity in different kinds of anticoagulant (EDTA, Oxalate, Heparin) while there was increase rhodanese activity when using citrate salts at p < 0.01 . Maximum enzyme activity was obtained using (50 µl) of plasma in one minute when incubated at (50ہC) using (125 µmol) of sodium thiosulphate and (150 µmol) potassium cyanide as a substrate. There was high inhibition rate by using calcium and sulphite salts compared to formaldehyde . It has been found that the normal range of Rhodanese activity in plasma according to optimum condition was (98.5±4.5 U/ml) and there was no significant difference in rhodanese activity in sex different while there was a significant increase in enzyme activity related to age at p < 0.01 . | ||
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