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استخلاص وتنقية انزيم بيتا لاكتميز من العزلتين المحليتين Proteus mirabilis 4TF, 20TF | ||
| Baghdad Science Journal | ||
| Article 1, Volume 6, Issue 2, 2009, Pages 249-256 | ||
| Authors | ||
| ; a S. Abbas; Hadi R.R. Al-Taai; Essam F. Al-Jumaily; Hussan H. Khanaeqah | ||
| Abstract | ||
| Proteus mirabilis β -lactamase of local isolates number 4TF represent karkh side and 20TF represent rusafa side of Baghdad were extracted and purified 23.17, 25.23 fold with yield of 36.66 %, 37.5% and specific activity 11.8, 12.6 of unit/ mg protein by DEAE –cellulose and Sepharose 4B (respectively ).Molecular weight of both enzyme was about 35500 Dalton determined by gel filtration. The study indicated that the isoelectric point of purified β -lactamase that extracted from isolate number 4TF and 20TF was 5.4. | ||
| Keywords | ||
| lactamase; Proteus mirabilis; Gel filtration; molecular; isoelectric point; Proteus mirabilis | ||
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