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Study the optical condition and effect of Ultraviolet ray on extracellular polysaccharide production from local isolation of Bacillus subtilis bacteria | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 15, Issue 2, 2010, Pages 181-186 | ||
| Abstract | ||
| The effect of Ultra violet (UV) different concentration of carbon and nitrogen sources, yeast extract and pH values on the ability of extracellular polysaccharide production in Bacillus subtilis were studied. The higher value of polysaccharide production is 5.15 g/l after 7 days from incubation when the bacteria were exposed to UV for 60min. Different carbon and nitrogen sources were added to the media. higher production of polysaccharide were found after adding sucrose and sodium nitrate to the media (5.63 , 5.10 g/L), respectively. Also, adding lactose and sodium nitrate showed decreasing in polysaccharide production (1.70 and 1.99 g/l), respectively. Supplementing the media with yeast extract at 2 g/l revealed increasing in polysaccharide production (5.41 g/l). Also, adjusting the pH value of the media to (5.5) showed increasing in polysaccharide production | ||
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