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Production of Polymyxin B Antibiotic from Bacillus sp. | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 3, Issue 2, 2011, Pages 16-26 | ||
| Abstract | ||
| Polymyxin antibiotic was isolated as an active wide spectrum as an oleic withes powder from Bacillus sp.The physiochemical properties of the extracted antibiotic were determined using thin layer chromatography (TLC), Infar Red Spectroscopy in addition to of qualification and color reactions.The extracted antibiotic was identified by using Gas Chromatographic technique to determine its chemical compound such as main groups of fatty acids and amino acids. The results show that the extracted antibiotic was Polymyxin B.The biological activity of the extracted antibiotic against the reference strains Staphylococcus aureus ATCC 25923 Escherichia coli ATCC 25922 Pseudomonas aeruginosa ATCC , 27859 and Klebsiella sp , was determined and the results showed that extracted Polymyxin B has the ability to inhibit the growth all types of reference stains in both primary and secondary screening . | ||
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