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Isolation and Identification of an Anthocyanin Compound from Cherry Fruit (Prunus Avium L.) and Study of its Antibacterial Activity | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 16, Issue 2, 2011, Pages 26-30 | ||
| Author | ||
| Mohammed A. Hussain | ||
| Abstract | ||
| This work includes the isolation and identification of cyanidin-3-glucoside as major anthocyanin in Iraqi Kurdistan cherry fruit (Prunus Avium L.). The extracted pigments were purified using physical methods including different chromatographic techniques (thin layer chromatography (TLC), liquid chromatography (LC) and high performance liquid chromatography (HPLC)). The pure extracted pigments were identified using different techniques such as infrared (IR), Ultraviolet (UV), H1-NMR and C13-NMR. The antibacterial activity of the extracted anthocyanin compound (cyanidin-3-glucoside) was determined against types of standard strains of bacteria which were Staphylococcus aureus (gram +ve) and Escherichia coli (gram –ve) by disk diffusion method the results indicates that the anthocyanin compound has inhibited the growth of Escherichia coli. | ||
| Keywords | ||
| cherry fruit; natural pigment; isolation; identification; anthocyanin; antibacterial activity; gram | ||
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