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Simple salting – out method for genomic DNA extraction from whole blood | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 16, Issue 2, 2011, Pages 9-11 | ||
| Author | ||
| Jawdat N. Gaaib | ||
| Abstract | ||
| The study conducted in the genetic engineering laboratory ,Biology department,College of Science,University of Tikrit,to modify and optimize DNA extraction protocol passed on salting out method, it carried out at the department of biology, college of science, Tikrit university from November 2009 to April 2010. Ten blood samples were collected in EDTA tube and transferred to a laboratory for DNA extraction. The DNA was extracted by use of modified salting out method . The quality and quantity of extracted DNA was measured spectrophotometricaly at wave length 260nm and 280 nm. The result showed that this method produces good quality high molecular weight DNA, also it was simple, rapid, minimum cost laboratory method. | ||
| Keywords | ||
| salting; out; DNA Extraction; blood | ||
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