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Isolation and determination of the approximately molecular weight of the active proteinous compounds from Soybean seeds and studying their effects in normal , alloxan-induced diabetic mice | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 16, Issue 2, 2011, Pages 128-138 | ||
| Author | ||
| Hamza Namik Hamead AL-Hussieny | ||
| Abstract | ||
| Dept. of Chemistry, College of Education, University of Mosul, Mosul, Iraq his study was designed to isolate and study the proteinous compounds from cold and boiled aqueous extract of Soybean plant seeds using different biochemical techniques, and including precipitation of the proteinous part in each of the cold boiled aqueous extract using cold acetone. Using gel filtration technique , two compounds (A and B) from the proteinous precipitate of the cold and boiled aqueous extract of Soybean plant seeds were isolated. The approximate molecular weights for these compounds were determined using gel filtration technique. The approximate molecular weight for A and B which were isolated from cold aqueous extract were (73400) and (7400) dalton respectively, and the boiled extract were (69900) and (6900) Dalton. The effects of cold , boiled proteinous precipitate obtained by acetone precipitation, were studied in normal , diabetic male mice The extracts were administrated intraperitoneally and their effects were compared with insulin. After one week of treatment the results indicated that proteinous precipitate cold and boiled, at the doses of 12.5 17.45 mg/Kg body weight respectively caused a significant decrease in serum glucose , triglycerid (TG) levels and a significant increase in serum high density lipoprotein cholesterol (HDL-C) in normal and alloxan diabetic mice. The proteinous compound A which was isolated from cold precipitate at the dose (3.6) mg/Kg of body weight showed a decreasing effect in serum (glucose, T. Cho, TG) levels, and a significant increase in HDL-C level in diabetic mice. Furthermore this compound produce also a biggest decrease effects in total cholesterol (T.Col) level in, diabetic mice. At the same time this compound A reaching the level of T. Cho in diabetic of male mice was similar to insuline. | ||
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