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Test of geuotoxicity of herbicid paraquat using Chromosome aberration in anaphase- telophase in Alium cepa | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 16, Issue 1, 2011, Pages 168-176 | ||
| Author | ||
| Wajdi Sabeeh Sadek | ||
| Abstract | ||
| Genotoxic effects was evaluated for the herbicide Paraquat in the meristem cells of Allium cepa. The value of EC50 was determined in the root growth test. Experiments was conducted for the concentrations (EC50/2), (EC50) and (EC50× 2) at 24, 48, 72 and 96 h with control for each group. Mitotic index were reduced with herbicide concentration at any time of exposure. The percentage of Sickness, bridges, vagrant chromosomes, c- anaphase, multipolarity, and fragments in anaphase- telophase cells according to the total cells with chromosome aberration scored was 38.87, 28.48, 16.67, 14.10, 1.60, and 0.64% respectively. Chromosome aberration increased with paraquat concentratin. Cells with micronuclei was observed at interphase cells. The higher frequency of micronuclei was at the concentration 5ppm of paraquat compared with other tested concentrations. | ||
| Keywords | ||
| Paraquat; Allium cepa | ||
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