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Novel Chemiluminometric-FIA Method for The Determination of DL-Histidine Via Loaded Ion Exchange Resin with Cobalt(II) Ion | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 16, Issue 1, 2011, Pages 31-37 | ||
| Author | ||
| Issam M.A. Shakir | ||
| Abstract | ||
| This research work presents completely new approach for the determination of DL-Histidine in a sensitive easy method. It relies on the use of cation exchange resin loaded with Co(II) ion. DL-Histidine replaces Co(II) ion entrapped in the resin bed structure giving to a release of Co(II) ion. The cobalt (II) ion catalyses the peroxide decomposition leading to a chemiluminescence reaction. The energy of emitted photons is measured and related to the concentration in a single well defined profile. The linearity for the 0.0-1000 mol.L-1 has correlation coefficient 0.9935, and a coefficient of determination 98.71% with a limit of detection of 50 nmol.L-1 using 25L sample. While using quadratic regression gave a correlation coefficient for 0.0-1000 mol.L-1 of 0.9981 with a coefficient of determination 99.63%. further treatment of data were handled and ANOVA for both equations were conducted which proves that both equations can be used for the range given above. Also a chromatographic calculation approach was conducted for assumed theoretical plates and HETP. Also Hex was calculated at approximatly Dm = 1. as an approximation. The method is at further detailed study. | ||
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