Effect of Lipid Rancidification on Some Antioxidants in  Albino Males Rats

H.Jankeer, Muna

	Effect of Lipid Rancidification on Some Antioxidants in Albino Males Rats
Tikrit Journal of Pure Science
Article 1, Volume 14, Issue 1, 2009, Pages 194-202
Author
Muna H.Jankeer
Abstract
The present study included the recognition which lipids could be oxidized (rancidity) when exposed to different factors such as high temperature, moisture, light and air during storage, as well as studying the effect of the ingestion of rancid lipid by mammalians on the non-enzymatic antioxidant existing in the body such as glutathione (GSH) level and enzymatic antioxidant like Superoxide dismutase (SOD) in the serum and tissue, taking into consideration the study of Malondialdelyde (MDA) level which is accounted as an indicator for the process of lipids rancidity in the serum and tissues. The first axis of the study included the exposure of animal lipids to various conditions as to temperature at (40)C and (50)C,moisture at a rate of (4%) and (6%) separately, as well as the other factors time, aeration and light. the effect of these conditions have been studied on the speed of lipids rancidity using a storage period of a (90) days as a model , through which chemical measurements of the peroxide values, iodine number and the acidic value have been performed every (10) days for the stored lipids. An increase in the speed of lipids rancidity stored at (50)C more than those stored at (40)C was noted and an increase was also noted in samples at a moisture rate of (4%) more than those stored at (6%). The second axis of the study included the preparation of typical forages for adult male rat. Which were divided into three groups; the first group has been fed with a forage containing an unprocessed lipids (the control); whereas the second group has been fed with a forage containing a rancid lipid which was stored at moisture rate of (4%). The third group had been fed with a forage containing a rancid lipid which was stored at (50) C. Feeding program was continued for (6) weeks. The third axis of the study includes determination of some of the antioxidants in the serum. The results showed a significant decrease in glutathione level and the level of superoxide dismutase and a significant decrease in malondialdelyde level in serum. The result of test on tissues showed a significant decrease in glutathione level accompanied with a significant increase in malondialdelyde level in the tissues of the heart, liver and kidney that fed on lipid rancid compared with the control group

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