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The Mutation Effect of Captan and Tolclofos-methyl in the Fungus Trichoderma viride For the Production of Cellulase and Single Cell Protein | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 14, Issue 1, 2009, Pages 134-143 | ||
| Author | ||
| Raghad Nabhan Al-Azzo | ||
| Abstract | ||
| This study was conducted aiming to obtain an efficient fungal isolate in cellulase and single cell protein production from a local isolate Trichoderma viride by the mutation effect of Captan and Tolclofos-methyl. It was found that Captan has a lethal effect on the isolate at all concentrations of the fungicide and spores suspension used, at the same time it was found that Tolclofos-methyl has an obvious mutational effect on the parental isolate at the concentration 2.5 mg active material/ml of standard medium, the parental isolate showed clear morphological changes in colony shapes, colors, ability for growth in addition to the profound changes in fungal mycelia which was confirmed by the application of slide culture technique. A qualitative and quantitative tests have been carried out to explore the efficiency of the new mutant for cellulase and single cell protein production in solid and liquid media, it achieved cellulase specific activity and single cell protein (7.158 micromole/min/mgm protein and 37.689 % protein in dry weight) respectively. | ||
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