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EVALUATION OF BAGHDAD STAIN FOR BLOOD AND BONE MARROW FILMS FOR STAINING THE CHROMOSOMES | ||
| AL-TAQANI | ||
| Article 1, Volume 21, Issue 3, 2008, Pages 0-0 | ||
| Abstract | ||
| An attempt was made to study the efficiency of Baghdad stain which is routinely used in blood and bone marrow films in staining the chromosomes. A total of 20 slides of cells of bone marrow films were flooded with the solution of Baghdad stain, then these slides were divided in to 4 groups and 5 slides in each group. Staining periods after dilution of the stain with distilled water for five slides were as follows: 5,7,10,15, and 20 minutes. Two slides were stained with Giemsa stain for control. Best result in staining of the chromosomes with Baghdad stain showed with 10 minutes staining period. In addition to the efficiency of Baghdad stain in staining the chromosomes, it has simple staining procedure, and easy method of preparation. According to these characteristics it can be suggested that Baghdad stain for blood and bone marrow films is recommended for staining the chromosomes | ||
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