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Initiation and Diffrentiation Of Coriandrum sativum L. Callus and its antimicrobial activity | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 13, Issue 1, 2008, Pages 62-69 | ||
| Author | ||
| Seham Ahmad Mahmood | ||
| Abstract | ||
| In this study callus was initiated from different explants (Leaves, stems, hypocotyles,cotyledones and roots) of Coriandrum sativum plants in Solidified Murashige and Skoog (MS ) medium . supplemented with different concentrations of (NAA ,BA), (IAA ,Kin) (2,4-D ,BA) and (2,4 –D , Kin ) . It was showen that solidified (MS ) medium containing 1.0 mg L BA and 0.5 mgL NAA was the best one for callus initiation and maintenance subculturing . Callus initiated from different explants except roots have the ability to produce shoots and stem callus gave the highest ratio of regeneration then the hypocotyls callus ,and callus of cotyledones and finally leaves Callus . In addition solidified MS medium supplemented with 1.0 mg L BA and 0.1 mg L NAA was the most suitable for shoots formation . All shoots were rooted in solidified half strength MS medium containing 0.5 mg L 2,4 –D . Also in this study the effect of alcoholic extract of leaves of seed born plants and leaves Callus was studied against Staphylococcus aureus , Bacillus subtilis , Escherichia coli , Pseudomonas aeroginosa by using sensitivity test . It was shown that the extracts have an inhibitory effect which differs according to the type and concentration of the extracts and also the type of bacteria. | ||
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