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EXTRACTION AND PURIFICATION OF ALPHA- HEMOLYSIN WHICH IS PRODUCING BY STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT CLINICAL SAMPLES | ||
| AL-TAQANI | ||
| Article 1, Volume 21, Issue 3, 2008, Pages 8-17 | ||
| Author | ||
| Talib Falih Hassen | ||
| Abstract | ||
| Four hundered seventy six clinical samples were collected from patients since June 2003 to May 2004 .The number of Staphylococcus aureus isolated from the samples was 163 isolates as follows : urine 52 isolates , wound swab 38 isolates , ear swab 21 isolates , tonsil swab 17 isolates. Boil swab 11 isolates and Nasal swab 24 isolates Alpha hemolysin of Staphylococcus aureus isolates was designated by hemolysin assay It was found that 42 % ( 70 ) of isolates were alpha - hemolysin producers. The extraction of alpha- hemolysin from liquid media from producing isolates was done by cooling centrifugation and purified by ammonium sulphate precipitation and gel - filtration chromatography. Activities of purified alpha - hemolysin were detected , it had 2560 HU/ ml the optimum temperature of alpha - hemolysin activity was 20-40 C and the optimum incubation period was 14hr. The optimum pH of alpha-hemolysin activity was 7.Cytotoxic activity of alpha–hemolysin against human leukocytes were determined and found the survival ratio of human leukocytes was decreased (38%)after treatment with 15 mg/ml for 30 min.while it was lost after treatment with 30 mg/ml for30 min. too. | ||
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