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A PROTOCOL FOR SHOOT REGENERATION FROM LEAVES PETIOLES TISSUE CULTURE OF NEEM TREES ( Melia azedarach ) | ||
| Mesopotamia Journal of Agriculture | ||
| Article 3, Volume 34, Issue 1, 2006, Pages 9-15 PDF (0 K) | ||
| DOI: 10.33899/magrj.2006.38498 | ||
| Author | ||
| Mozahim K. AL- Mallah | ||
| Abstract | ||
| Shoots were regenerated from petioles – derived callus on solidified Murashige and Skoog (MS) medium supplemented with 1.5 mg / l BA only and MS medium containing 4.0 mg / l IBA and 1.0 mg / l BA . The percentage of shoot formation was 30.0 % . The regeneration frequency was reasonable to obtained sufficient shoots by this method . This simple protocol is the first report in tissue culture of this woody plant which encourage other researchers to worke other studies in tissue culture of neem . Moreover , callus culture will be useful to obtain a wide range of industrial plant products . | ||
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