COMBINED MATING TECHNIQUE  FOR ASSESSING CONJUGAL TRANSFER BETWEEN TEST VIBRIO CHOLERAE 01 MZW 41  AND PSEUDOMONAS AERUGINOSA W 109

Ahmed Al, Wiaam; Amili,

	COMBINED MATING TECHNIQUE FOR ASSESSING CONJUGAL TRANSFER BETWEEN TEST VIBRIO CHOLERAE 01 MZW 41 AND PSEUDOMONAS AERUGINOSA W 109
AL-TAQANI
Article 1, Volume 21, Issue 3, 2008, Pages 54-62
Authors
Wiaam Ahmed Al;
Abstract
This study presents results of comparing four in vitro conjugal transfer techniques, colony cross streak (CCS), broth mating (BM), combined spread plate (CSP), and membrane filtration (MF) with each other in evaluating the transfer of cojugative plasmids. Vibrio cholerae 01 MZW 41 and Pseudomonas aeruginosa W109, were used as mating pairs. The suitability of each method was evaluated for use in a routine assessment of the genetic stability of genetically transconjugated microorganism. By the BM technique, transconjugants were usually produced in 100% of the mating traits. In general, differences in the percentage of successful mating show that BM technique is statically significant at the P ≤ 0.05 level, compared with CCS, CSP, and MF techniques. Since the genetic exchange between Vibrio and Pseudomonas was not detected previously (an exception to this, what has been reported by Al – Amili, W.A. (2001), and any single conjugation transconjugants. We have used a combined mating technique which integrates the CCS, CSP, BM, and MF method as a single procedure to assess the mobility of plasmid DNA of genetically transconjugated microorganisms

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