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Cytosine deaminase activity in earthworm Lumbricus terrestris (Linnaeus,1758) | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 12, Issue 1, 2007, Pages 22-25 | ||
| Author | ||
| Zahra I. Dallal Bashi | ||
| Abstract | ||
| Cytosine deaminase activity was found in the supernatant fraction of earthworm Lumbricus terrestris extract (Linnaeus,1758), and some of its properties were investigated. Maximum enzyme activity was obtained in a reaction mixture of (200)mM of potassium phosphate buffer at pH (6.0) containing (3)µM of cytosine as a substrate, (60-65)mg protein as a source of the enzyme, and an incubation period of (20) min. at (25)°C. Under the optimum conditions, the specific activity was found to be(22.16)mM of cytosine deaminated per min per mg | ||
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