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Isolation of extracellular protease from Trichophyton mentagrophytes of Skin isolates | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 14, Issue 3, 2009, Pages 35-41 | ||
| Author | ||
| Ayhan R. Mahmood | ||
| Abstract | ||
| Dermatophyte fungi of the genera Trichophyton, Epidermophyton, and Microsporum infect human skin, hair, and nails. These types of infections, termed "dermatophytoses," are widespread that affects billions of individuals worldwide. In the present study, we isolated Trichophyton mentagrophytes from patients with dermatophytoses. The isolates were grown on Sabouraud agar and were identified on the basis of microscopic examination, for the presence of fungal elements. To promote production of protease, T. mentagrophytes isolate cultured in protein liquid medium. Results showed that T. mentographytes have ability to produce extracellular protease which indicated by proteolysis activity 111.9 U/ml against casein when added as substrate to the culture filtrates of T. mentographytes with specific activity 2238U/mg. To optimize protease production by T. mentographytes, the effect of different cultural conditions (production media, incubation period, optimal temperature and pH) on production of extracellular protease were studied. The results showed that T. mentographytes have high ability to produce protease after 9 days of incubation in protein liquid media at 30 °C, and pH 7.0. The extracellular protease synthesized by T. mentagrophytes extracted, then partial purified by using Fractionation by Aqueous Two Phase System. | ||
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