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Biotyping and using of ELISA for detection of listeria monocytogenes isolated from poultry and aborted women | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 14, Issue 3, 2009, Pages 8-12 | ||
| Authors | ||
| ; I. A. Al | ||
| Abstract | ||
| Listeria monocytogens was isolated from 50 blood samples of aborted women and 75 blood samples of poultry (broiler). The organism was diagnosed by cultured and biochemical methods and confirmed serologically, using Enzyme-Linked Immunosorbant Assay (ELISA). A cording to culture result, 18 (36%) isolates were for blood samples of aborted women, while 15 (20%) isolates from blood samples were recorded in poultry. Serologically, using ELISA with the ListerioLysin O (LLO) as antigen, there were 12 (24%) isolates from aborted women, and isolates from poultry 9 (12%). Biotyping of the organism showed that the isolates were belonged to 7 biotypes, depending on their ability to produce Lecithinase, Estrase and DNase production. The study, also, showed that aborted women and poultry isolates were occurred in the I, II, and V biotypes at percentages rates of 28.5%, 19% and 23.8%, respectively. | ||
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