A.alaubydi, M., K.M, A. (2010). Extraction and partial purification for adhesive protein FimH from type-1 pili which isolated from uorpathogenic E.coli. , 7(0), 327-333.
Mouruj A.alaubydi; Alice K.M. "Extraction and partial purification for adhesive protein FimH from type-1 pili which isolated from uorpathogenic E.coli". , 7, 0, 2010, 327-333.
A.alaubydi, M., K.M, A. (2010). 'Extraction and partial purification for adhesive protein FimH from type-1 pili which isolated from uorpathogenic E.coli', , 7(0), pp. 327-333.
A.alaubydi, M., K.M, A. Extraction and partial purification for adhesive protein FimH from type-1 pili which isolated from uorpathogenic E.coli. , 2010; 7(0): 327-333.

Extraction and partial purification for adhesive protein FimH from type-1 pili which isolated from uorpathogenic E.coli

Baghdad Science Journal
Article 1, Volume 7, Issue 0, 2010, Pages 327-333
Authors
Mouruj A.alaubydi; Alice K.M
Abstract
Type-1 fimbriae have been extracted from E.coli which was previously isolated, the yield was 2.8 mg fimbriae/10 g bacteria , the crude fimbriae was partially purified by several solubilization and recrystilization methods using a series of buffer and solutions to decrease the impurities, the yield of fimbriae from all these steps was 2.1 mg fimbriae / 10 g bacteria.
Adhesive protein FimH was extracted from fimbriae by separation then partially purified by Gel-chromatography sephadex G-100, the yield of FimH was 250 µg/ml, while fimbriae protein was 50.6 µg/ml. The partially purified FimH revealed one clear band in SDS-PAGE, as well as protein fimbriae with molecular weight (28300,17500) Dalton respectively, when compared with standard protein curve.
Keywords
coli
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